Protocol for Serial Block-Face Scanning Elect | JoVE Journal
Protocol for Serial Block-Face Scanning Elect | JoVE Journal
Sep 25, 2024
jove.com
Protocol for Serial Block-Face Scanning Elect | JoVE Journal
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Protocol for Serial Block-Face Scanning Elect | JoVE Journal
8.6K viewsMar 26, 2021
jove.com
Serial block-face scanning electron microscopy (SBF-SEM) utilizes an ultramicrotome inside the SEM chamber to image the ultrastructure of a resin-embedded biological sample in 3D over a large area. A diamond knife slices sections from the sample block and the exposed sample surface is imaged by the electron beam and backscatter electron detector. The cutting and imaging process is automatically repeated until the desired – or entire - stack is acquired in the z direction of the sample. The indiv
0:58
Serial block-face scanning electron microscopy (SBF-SEM) utilizes an ultramicrotome inside the SEM chamber to image the ultrastructure of a resin-embedded biological sample in 3D over a large area. A diamond knife slices sections from the sample block and the exposed sample surface is imaged by the electron beam and backscatter electron detector. The cutting and imaging process is automatically repeated until the desired – or entire - stack is acquired in the z direction of the sample. The indiv
571 viewsMay 22, 2023
FacebookZEISS Microscopy
We're excited to show the ZEISS Volutome for Serial Block-Face SEM at #mmc2023 and we're not alone - Dr. Ian White is looking forward to it too! Book a demo today and visit us at stand 111 from 4th - 6th July - https://zeiss.ly/mmc2023 See you there! #microscopy #sem | ZEISS Microscopy
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We're excited to show the ZEISS Volutome for Serial Block-Face SEM at #mmc2023 and we're not alone - Dr. Ian White is looking forward to it too! Book a demo today and visit us at stand 111 from 4th - 6th July - https://zeiss.ly/mmc2023 See you there! #microscopy #sem | ZEISS Microscopy
294 viewsJun 28, 2023
FacebookZEISS Microscopy
In today's Wiley webinar, explore the power of serial block-face imaging: a key technology in the Volume EM revolution. See its applications in life science research and how it is used in core facilities. Join Prof. Fengxia Liang from the Department of Cell Biology at NYU Langone Health, and Aubrey Funke, Product Marketing Manager Life Science, ZEISS Microscopy, at 11 am EST or 9 pm EST today. Register here: https://zeiss.ly/fb-o-sbf-sem-wiley-webinar-23 #VolumeEM #vEM #electronmicroscopy #micro
0:23
In today's Wiley webinar, explore the power of serial block-face imaging: a key technology in the Volume EM revolution. See its applications in life science research and how it is used in core facilities. Join Prof. Fengxia Liang from the Department of Cell Biology at NYU Langone Health, and Aubrey Funke, Product Marketing Manager Life Science, ZEISS Microscopy, at 11 am EST or 9 pm EST today. Register here: https://zeiss.ly/fb-o-sbf-sem-wiley-webinar-23 #VolumeEM #vEM #electronmicroscopy #micro
374 viewsJun 27, 2023
FacebookZEISS Microscopy
【DISCONTINUED】Serial Block-face SEM JSM-7200F・7800F / Gatan 3View®2XP | Products | JEOL Ltd. | Products | JEOL Ltd.
【DISCONTINUED】Serial Block-face SEM JSM-7200F・7800F / Gatan 3View®2XP | Products | JEOL Ltd. | Products | JEOL Ltd.
Oct 4, 2022
jeol.com
1,348 Serial cross-sectional images of resin-embedded white LED phosphors were automatically acquired using Serial Block Face SEM. UVD images depicted phosphors as white particles while resin as black background. 3D reconstruction using an image processing software, Image-Pro, revealed phosphor shape, size, and distribution. Green-colored particles have characteristic shapes (60 mm or more in outside diameter). #LED #WhiteLED #Phosphor #SerialBlockFace #SEM #ElectronMicroscopy #ElectronMicroscop
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1,348 Serial cross-sectional images of resin-embedded white LED phosphors were automatically acquired using Serial Block Face SEM. UVD images depicted phosphors as white particles while resin as black background. 3D reconstruction using an image processing software, Image-Pro, revealed phosphor shape, size, and distribution. Green-colored particles have characteristic shapes (60 mm or more in outside diameter). #LED #WhiteLED #Phosphor #SerialBlockFace #SEM #ElectronMicroscopy #ElectronMicroscop
77 viewsMar 4, 2025
FacebookHitachi Electron Microscope
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At the BIC, Fabrice Cordelières & team transformed microscopy data into something you can hold: a 3D-printed trypanosome!Reconstructed from SBF-SEM image stack, this parasite becomes a tangible model!In parallel, SEM images provide a 2D visualization of the surface topography
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